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qARTA Bio Products

q•Hot Taq Kits

Catalog No. Description Volume Size
QHB1-500 q•Hot Taq with 10X Buffer B1 100 uL 500 U
QHB2-500 q•Hot Taq with 10X Buffer B2 100 uL 500 U
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Product Detail
Reaction Mix
Protocol
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q•Hot Taq Kits include q•Hot Taq which is a chemically modified Hot Start DNA Polymerase, 10X reaction buffer B1 or B2, 25mM MgCl2, and 10X Enhancer for GC-rich PCR. q•Hot Taq has 5’->3’ DNA polymerase activity, 5’->3’ exonuclease activity, and generates PCR products with 3’-dA overhangs.

Recommended Reaction Mix

Recommended PCR Mix:

Component Concentration Volume Final Concentration
q•Taq 5 U/µL 0.2 - 0.5 µL 1 - 2.5 U
q•Buffer A or B 10X 10 µL 1X
>MgCl2 25 mM 3 - 5 µL 1.5 - 2.5 mM
dNTP mix 20 mM 0.5 µL 0.2 mM
Primer, forward 10 pmol/µl 0.5 - 1.5 µL 100 - 300 nM
Primer, reverse 10 pmol/µl 0.5 - 1.5 µL 100 - 300 nM
Template DNA 5 - 500ng 0.1 - 10 ng/µL
Enhancer 10X 0 - 15 µL 0 - 3X
Nuclease-free water variable
Total 50 µL

For complete usage instructions download full User Protocol.

Recommended Instrument Protocol

Recommended Instrument Protocol:

Cycle Step Temperature Time Cycles
Initial denaturation 95°C 12 - 15 min 1
Denaturation 95°C 30 - 60 s 26 - 35
Annealing 50-68°C 30 - 60 s
Extension 72°C 1 - 4 min
Final Extension 72°C 5 - 10 min 1

For complete usage instructions download full User Protocol.


User Protocol
MSDS
CofA

  • Environmentally green: No shipping on ice required. Reduces packaging waste by 75%!

  • Nuclease-free Water Included: All mixes include sufficient RNase and DNase Free water for recommended reaction volume.
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