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qARTA Bio Products

q•Taq Kits

Catalog No. Description Volume Size
QTA-500 q•Taq with Buffer A 100 µL 500 U
QTB-500 q•Taq with Buffer B 100 µL 500 U
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Product Detail
Reaction Mix
Protocol
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q•Taq is a thermostable DNA polymerase. Due to its genetic modifications q•Taq has an enhanced stability at ambient temperature. The enzyme has 5’-> 3’ polymerization-dependent exonuclease replacement activity but lacks 3’-> 5’ exonuclease activity.

Recommended Reaction Mix

Recommended PCR Mix:

Component Concentration Volume Final Concentration
q•Taq 5 U/µL 0.4 - 1.0 µL 2 - 5 U
q•Buffer A or B 10X 10 µL 1X
>MgCl2 25 mM 6 - 10 µL 1.5 - 2.5 mM
dNTP mix 20 mM 1 µL 200 µM of each dNTP
Primer, forward 10 pmol/µl 1 - 3 µL 100 - 300 nM
Primer, reverse 10 pmol/µl 1 - 3 µL 100 - 300 nM
Template DNA 1 - 5 µL 5 - 100 ng/µL
Enhancer 10X 0, 10, 20, or 30 µL 1X, 2X, or 3X
Nuclease-free water variable
Total 100 µL

For complete usage instructions download full User Protocol.

Recommended Instrument Protocol

Recommended Instrument Protocol:

Cycle Step Temperature Time Cycles
Initial denaturation 95°C 3 - 5 min 1
Denaturation 95°C 30 - 60 s 26 - 35
Annealing 60-65°C 30 - 60 s
Extension 72°C 20 s
Final Extension 72°C 5 - 10 min 1

For complete usage instructions download full User Protocol.


User Protocol
MSDS
CofA

  • Environmentally green: No shipping on ice required. Reduces packaging waste by 75%!

  • Nuclease-free Water Included: All mixes include sufficient RNase and DNase Free water for recommended reaction volume.
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